A covalently bound photoisomerizable agonist. Comparison with reversibly bound agonists at electrophorus electroplaques

After disulphide bonds are reduced with dithiothreitol, trans-3-(alpha-bromomethyl)-3’-[alpha-(trimethylammonium)methyl]azobenzene (trans-QBr) alkylates a sulfhydryl group on receptors. The membrane conductance induced by this “tethered agonist” shares many properties with that induced by reversible agonists. Equilibrium conductance increases as the membrane potential is made more negative; the voltage sensitivity resembles that seen with 50 [mu]M carbachol. Voltage- jump relaxations follow an exponential time-course; the rate constants are about twice as large as those seen with 50 mu M carbachol and have the same voltage and temperature sensitivity. With reversible agonists, the rate of channel opening increases with the frequency of agonist-receptor collisions: with tethered trans-Qbr, this rate depends only on intramolecular events. In comparison to the conductance induced by reversible agonists, the QBr-induced conductance is at least 10-fold less sensitive to competitive blockade by tubocurarine and roughly as sensitive to “open-channel blockade” bu QX-222. Light-flash experiments with tethered QBr resemble those with the reversible photoisomerizable agonist, 3,3’,bis-[alpha-(trimethylammonium)methyl]azobenzene (Bis-Q): the conductance is increased by cis {arrow} trans photoisomerizations and decreased by trans {arrow} cis photoisomerizations. As with Bis-Q, ligh-flash relaxations have the same rate constant as voltage-jump relaxations. Receptors with tethered trans isomer. By comparing the agonist-induced conductance with the cis/tans ratio, we conclude that each channel’s activation is determined by the configuration of a single tethered QBr molecule. The QBr-induced conductance shows slow decreases (time constant, several hundred milliseconds), which can be partially reversed by flashes. The similarities suggest that the same rate-limiting step governs the opening and closing of channels for both reversible and tethered agonists. Therefore, this step is probably not the initial encounter between agonist and receptor molecules

A photoisomerizable muscarinic antagonist. Studies of binding and of conductance relaxations in frog heart

These experiments employ the photoisomerizable compound, 3,3'-bis- [alpha-(trimethylammonium)methyl]azobenzene (Bis-Q), to study the response to muscarinic agents in frog myocardium. In homogenates from the heart, trans-Bis-Q blocks the binding of [3H]-N-methylscopolamine to muscarinic receptors. In voltage-clamped atrial trabeculae, trans- Bis-Q blocks the agonist-induced potassium conductance. The equilibrium dose-response curve for carbachol is shifted to the right, suggesting competitive blockade. Both the biochemical and electrophysiological data yield a dissociation constant of 4-5 microM for trans-Bis-Q; the cis configuration is severalfold less potent as a muscarinic blocker. Voltage-clamped preparations were exposed simultaneously to carbachol and Bis-Q and were subjected to appropriately filtered flashes (less than 1 ms duration) from a xenon flashlamp. Trans leads to cis and cis leads to trans photoisomerizations cause small (less than 20%) increases and decreases, respectively, in the agonist-induced current. The relaxation follows an S-shaped time course, including an initial delay or period of zero slope. The entire waveform is described by [1 - exp(-kt)]n. At 23 degrees C, k is approximately 3 s-1 and n is 2. Neither k nor n is affected when: (a) [Bis-Q] is varied between 5 and 100 microM; (b) [carbachol] is varied between 1 and 50 microM; (c) carbachol is replaced by other agonists (muscarine, acetylcholine, or acetyl-beta-methylcholine); or (d) the voltage is varied between the normal resting potential and a depolarization of 80 mV. However, in the range of 13-30 degrees C, k increases with temperature; the Q10 is between 2 and 2.5. In the same range, n does not change significantly. Like other investigators, we conclude that the activation kinetics of the muscarinic K+ conductance are not determined by ligand-receptor binding, but rather by a subsequent sequence of two (or more) steps with a high activation energy

Classification of minimal actions of a compact Kac algebra with amenable dual

We show the uniqueness of minimal actions of a compact Kac algebra with amenable dual on the AFD factor of type II$_1$. This particularly implies the uniqueness of minimal actions of a compact group. Our main tools are a Rohlin type theorem, the 2-cohomology vanishing theorem, and the Evans-Kishimoto type intertwining argument.Comment: 68 pages, Introduction rewritten; minor correction

Canalizing Kauffman networks: non-ergodicity and its effect on their critical behavior

Boolean Networks have been used to study numerous phenomena, including gene regulation, neural networks, social interactions, and biological evolution. Here, we propose a general method for determining the critical behavior of Boolean systems built from arbitrary ensembles of Boolean functions. In particular, we solve the critical condition for systems of units operating according to canalizing functions and present strong numerical evidence that our approach correctly predicts the phase transition from order to chaos in such systems.Comment: to be published in PR

Lattice dynamics and structural stability of ordered Fe3Ni, Fe3Pd and Fe3Pt alloys

We investigate the binding surface along the Bain path and phonon dispersion relations for the cubic phase of the ferromagnetic binary alloys Fe3X (X = Ni, Pd, Pt) for L12 and DO22 ordered phases from first principles by means of density functional theory. The phonon dispersion relations exhibit a softening of the transverse acoustic mode at the M-point in the L12-phase in accordance with experiments for ordered Fe3Pt. This instability can be associated with a rotational movement of the Fe-atoms around the Ni-group element in the neighboring layers and is accompanied by an extensive reconstruction of the Fermi surface. In addition, we find an incomplete softening in [111] direction which is strongest for Fe3 Ni. We conclude that besides the valence electron density also the specific Fe-content and the masses of the alloying partners should be considered as parameters for the design of Fe-based functional magnetic materials.Comment: Revised version, accepted for publication in Physical Review

Cooling and heating by adiabatic magnetization in the Ni50_{50}Mn34_{34}In16_{16} magnetic shape memory alloy

We report on measurements of the adiabatic temperature change in the inverse magnetocaloric Ni$_{50}$Mn$_{34}$In$_{16}$ alloy. It is shown that this alloy heats up with the application of a magnetic field around the Curie point due to the conventional magnetocaloric effect. In contrast, the inverse magnetocaloric effect associated with the martensitic transition results in the unusual decrease of temperature by adiabatic magnetization. We also provide magnetization and specific heat data which enable to compare the measured temperature changes to the values indirectly computed from thermodynamic relationships. Good agreement is obtained for the conventional effect at the second-order paramagnetic-ferromagnetic phase transition. However, at the first order structural transition the measured values at high fields are lower than the computed ones. Irreversible thermodynamics arguments are given to show that such a discrepancy is due to the irreversibility of the first-order martensitic transition.Comment: 5 pages, 4 figures. Accepted for publication in the Physical Review

Uso da farinha de minhoca como alimento para pós-larvas de tilápia.

Foi avaliada a influência da substituição da farinha de peixe pela farinha de minhoca (Eisenia foetida) no crescimento de pós-larvas de tilápia nilótica (Oreochromis niloticus). A farinha de peixe, que correspondeu a 50% da proteína da dieta, foi substituída pela farinha de minhoca nos seguintes níveis: 0%, 20%, 40%, 60%, 80% e 100%. Os peixes foram alimentados à vontade, quatro vezes ao dia, sendo pesados e medidos aos 21 e 41 dias de experimentação. O delineamento experimental foi o completamente casualizado, com quatro repetições por tratamento e 20 peixes por unidade experimental. Os dados coletados foram analisados pela ANOVA, sendo as médias posteriormente classificadas pelo teste de Tukey (5%). Após 21 dias, não houve diferença significativa entre os tratamentos. Entretanto, aos 41 dias houve diferença significativa entre os tratamentos e os animais com o nível de substituição de 20% apresentaram os maiores pesos e taxas de crescimento específico, e os animais com o nível de substituição de 100% os menores. Durante o período experimental não houve diferença significativa entre os tratamentos em relação à sobrevivência dos animais. Os resultados mostram que baixos níveis de substituição da farinha de peixe (20%) melhoram o crescimento dos animais e que a substituição total da farinha de peixe pela farinha de minhoca é prejudicial ao desenvolvimento dos peixes, mas não afeta a sua sobrevivência.bitstream/item/37405/1/BP45.pd

Tilt effects on moment tensor inversion in the near field of active volcanoes

Dynamic tilts (rotational motion around horizontal axes) change the projection of local gravity onto the horizontal components of seismometers. This causes sensitivity of these components to tilt, especially at low frequencies. We analyse the consequences of this effect onto moment tensor inversion for very long period (vlp) events in the near field of active volcanoes on the basis of synthetic examples using the station distribution of a real deployed seismic network and the topography of Mt. Merapi volcano (Java, Indonesia). The examples show that for periods in the vlp range of 10-30 s tilt can have a strong effect on the moment tensor inversion, although its effect on the horizontal seismograms is significant only for few stations. We show that tilts can be accurately computed using the spectral element method and include them in the Green's functions. The (simulated) tilts might be largely influenced by strain-tilt coupling (stc). However, due to the frequency dependence of the tilt contribution to the horizontal seismograms, only the largest tilt signals affect the source inversion in the vlp frequency range. As these are less sensitive to stc than the weaker signals, the effect of stc can likely be neglected in this application. In the converse argument, this is not necessarily true for longer periods, where the horizontal seismograms are dominated by the tilt signal and rotational sensors would be necessary to account for it. As these are not yet commercially available, this study underlines the necessity for the development of such instrument

Hidden semi-Markov Model based earthquake classification system using Weighted Finite-State Transducers

Automatic earthquake detection and classification is required for efficient analysis of large seismic datasets. Such techniques are particularly important now because access to measures of ground motion is nearly unlimited and the target waveforms (earthquakes) are often hard to detect and classify. Here, we propose to use models from speech synthesis which extend the double stochastic models from speech recognition by integrating a more realistic duration of the target waveforms. The method, which has general applicability, is applied to earthquake detection and classification. First, we generate characteristic functions from the time-series. The Hidden semi-Markov Models are estimated from the characteristic functions and Weighted Finite-State Transducers are constructed for the classification. We test our scheme on one month of continuous seismic data, which corresponds to 370 151 classifications, showing that incorporating the time dependency explicitly in the models significantly improves the results compared to Hidden Markov Models